DJ.Studio is a DJ software application developed by DJ.Studio B.V., based in Leeuwarden in the Fryslân region of the northern Netherlands. The company was founded by Siebrand Dijkstra, who set out to build a modern, timeline-based alternative to legacy mix-creation tools like MixMeister after that program was discontinued. The software runs on macOS and Windows.

Rather than emulating a traditional turntable or CDJ setup, DJ.Studio takes a digital audio workstation approach to DJ mixing, letting users arrange full mixes, mashups, radio shows, and live sets on a timeline. Notable features include Harmonize, which orders playlists by tempo and harmonic compatibility using the Camelot Wheel; automated transitions with effects and automation lanes; stem separation for finer-grained editing; a music visualizer that exports MP4 video; and an accelerated 5x recording mode. The app integrates with libraries from rekordbox, Serato, Traktor, Virtual DJ, Engine DJ, and iTunes.

DJ.Studio won the NAMM TEC Award in the DJ Production Technology category and has built a user community in the hundreds of thousands. It is generally positioned as a tool for producing pre-planned mixes and mashups rather than for live club performance.

DJ.Studio has 6 products cataloged on Equipboard, including DJ Software and DAW Software.